ENPP1 (D37B7) Rabbit mAb #5342
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
ENPP1 (D37B7) Rabbit mAb detects endogenous levels of total ENPP1 protein.
Species Reactivity:
Human
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu520 of human ENPP1 protein.
Background
Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. Targeting of ENPP1 to the basolateral cell surface relies on the presence of a carboxy-terminal di-leucine-based signal (1). ENPP1 plays important roles in bone mineralization and soft tissue calcification (2-5). Mutations in the corresponding ENPP1 gene cause generalized arterial calcification in infancy (GACI) and idiopathic infantile arterial calcification (IIAC) (6,7). ENPP1 inhibits insulin receptor function and overexpression of this enzyme causes insulin resistance and glucose intolerance in mice (8,9). Genetic variants of ENPP1 have been associated with obesity and type 2 diabetes (10-12).
- Bello, V. et al. (2001) Mol Biol Cell 12, 3004-15.
- Okawa, A. et al. (1998) Nat Genet 19, 271-3.
- Nakamura, I. et al. (1999) Hum Genet 104, 492-7.
- Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.
- Hessle, L. et al. (2002) Proc Natl Acad Sci USA 99, 9445-9.
- Rutsch, F. et al. (2003) Nat Genet 34, 379-81.
- Ruf, N. et al. (2005) Hum Mutat 25, 98.
- Dong, H. et al. (2005) Diabetes 54, 367-72.
- Maddux, B.A. et al. (2006) Am J Physiol Endocrinol Metab 290, E746-9.
- Meyre, D. et al. (2005) Nat Genet 37, 863-7.
- Keene, K.L. et al. (2008) Diabetes 57, 1057-62.
- Bacci, S. et al. (2007) Curr Opin Clin Nutr Metab Care 10, 403-9.
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