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EpCAM (D9S3P) Mouse Chimeric Monoclonal Antibody #48769

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  • IHC

    Product Specifications

    REACTIVITY H M Mk
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse chimeric IgG2a
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as EpCAM (D9S3P) Rabbit Monoclonal Antibody #14452.

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Protocol

    Specificity / Sensitivity

    EpCAM (D9S3P) Mouse Chimeric Monoclonal Antibody recognizes endogenous levels of total EpCAM protein. Nonspecific staining was observed in monkey liver by immunohistochemistry.

    Species Reactivity:

    Human, Mouse, Monkey

    Source / Purification

    This recombinant chimeric antibody is engineered from EpCAM (D9S3P) Rabbit Monoclonal Antibody #14452 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, EpCAM (D9S3P) Rabbit Monoclonal Antibody #14452, is produced by immunizing animals with recombinant protein specific to human EpCAM protein.

    Background

    Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).

    Alternate Names

    ACSTD1; Adenocarcinoma-associated antigen; carcinoma-associated antigen GA733-2; CD326; Cell surface glycoprotein Trop-1; CO-17A; CO17-1A; DIAR5; EGP; EGP-2; EGP314; EGP34; EGP40; Ep-CAM; EPCAM; Epithelial cell adhesion molecule; Epithelial cell surface antigen; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733-2; hEGP-2; hEGP314; HNPCC8; human epithelial glycoprotein-2; KS 1/4 antigen; KS1/4; KSA; M1S2; M4S1; Major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); MIC18; MK-1; TACST-1; TACSTD1; TROP1; trophoblast cell surface antigen 1; Tumor-associated calcium signal transducer 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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