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88469
EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free) #88469

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  1. WB
  2. IHC
Western Blotting Image 1: EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Western blot analysis of extracts from various cell lines and tissues using EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded orthotopic 4T1 mammary tumor using EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) performed on the Leica® Bond Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded metastatic 4T1 tumor in mouse lung using EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) performed on the Leica® Bond Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse lung using EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse colon using EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse prostate using EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse small intestine using EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mIMCD-3 cell pellet (left, positive) or C2C12 cell pellet (right, negative) using EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred). Data were generated using the standard formulation of this product.
To Purchase # 88469
Cat. # Size Qty. Price
88469SF
100 µg
$ 746

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 35-45
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #93790. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #93790.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) (BSA and Azide Free) recognizes endogenous levels of total EpCAM protein. This antibody is preferred for immunohistochemical analysis of mouse tissues.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg311 of mouse EpCAM protein.

Background

Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).
  1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
  2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
  3. Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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