EpCAM (E6V8Y) XP® Rabbit mAb #93790
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H M R Hm |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35-45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| IHC Leica Bond | 1:75 - 1:300 |
| Immunohistochemistry (Paraffin) | 1:150 - 1:600 |
Storage
For a carrier-free (BSA and azide free) version of this product see product #88469.
Protocol
Specificity / Sensitivity
EpCAM (E6V8Y) XP® Rabbit mAb (Mouse Preferred) recognizes endogenous levels of total EpCAM protein. This antibody is preferred for immunohistochemical analysis of mouse tissues. For human tissue analysis, EpCAM (D9S3P) Rabbit mAb (IHC Preferred) #14452 is suggested.
Species Reactivity:
Human, Mouse, Rat, Hamster
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg311 of mouse EpCAM protein.
Background
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). Research studies identified EpCAM as one of the first tumor-associated antigens, and it has long been a marker of epithelial and tumor tissue. Investigators have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).
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