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14043
EPLIN Antibody
Primary Antibodies

EPLIN Antibody #14043

This product is discontinued

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# Product Name Application Reactivity
  • WB
  • IF
H M R
Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

EPLIN Antibody recognizes endogenous levels of total EPLIN protein, including EPLIN-α and EPLIN-β isoforms.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly450 of full-length human EPLIN-β protein, which corresponds to Gly290 of EPLIN-α protein. Antibodies are purified by protein A and peptide affinity chromatography.

Epithelial Protein Lost in Neoplasm (EPLIN) is an actin-binding protein that regulates actin filament dynamics and cross-linking (1). Alpha and beta isoforms are generated from alternate promoters, with the EPLIN-β isoform representing the full-length protein and the EPLIN-α isoform lacking the amino-terminal 160 amino acids (2). Increased expression of EPLIN protein results in more abundant and larger actin stress fibers due to stabilizing of cross-links and inhibition of actin depolymerization. EPLIN protein inhibits Rac1-promoted membrane ruffling and Arp2/3-associated actin filament branching (1).

Research studies demonstrate reduced EPLIN-α expression in tumor tissues, and correlate this reduction with increased invasiveness and poor clinical outcomes (3). The EPLIN protein is an important negative regulator of the epithelial-mesenchymal transition (EMT)(4). While EMT is a critical process during normal embryonic development, dysregulation in transformed cells is a key step in the transition to metastasis (5).

  1. Maul, R.S. et al. (2003) J Cell Biol 160, 399-407.
  2. Chen, S. et al. (2000) Gene 248, 69-76.
  3. Liu, Y. et al. (2012) Anticancer Res 32, 1283-9.
  4. Zhang, S. et al. (2011) Oncogene 30, 4941-52.
  5. Tsai, J.H. and Yang, J. (2013) Genes Dev 27, 2192-206.
Entrez-Gene Id
51474
Swiss-Prot Acc.
Q9UHB6
For Research Use Only. Not For Use In Diagnostic Procedures.

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