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9330
FAK Antibody Sampler Kit

FAK Antibody Sampler Kit #9330

Western Blotting Image 1

Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr576/577) Antibody (upper) or FAK antibody (lower).

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Western Blotting Image 2

Western blot analysis of extracts from Jurkat cells, untreated or anti-CD3 antibody-treated (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr925) Antibody (upper) or FAK antibody (lower).

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Western Blotting Image 3

Western blot analysis of extracts from A549 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb (upper) and FAK Antibody #3285 (lower).

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Western Blotting Image 4

Western blot analysis of extracts from various cell lines using FAK (D2R2E) Rabbit mAb.

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Western Blotting Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IP Image 6

Immunoprecipitation of FAK from U-87 MG cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or FAK (D2R2E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FAK (D2R2E) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-FAK (Tyr576/577) Antibody 3281 20 µl
  • WB
  • IP
H M R 125 Rabbit 
Phospho-FAK (Tyr925) Antibody 3284 20 µl
  • WB
  • IP
H M 125 Rabbit 
Phospho-FAK (Tyr397) (D20B1) Rabbit mAb 8556 20 µl
  • WB
  • IP
H 125 Rabbit IgG
FAK (D2R2E) Rabbit mAb 13009 20 µl
  • WB
  • IP
H M 125 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The FAK Antibody Sampler Kit provides an economical means of evaluating total FAK protein levels as well as FAK phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform two western blots with each antibody.

Each antibody in the FAK Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross react with other proteins. Activation state antibodies recognize target proteins only when phosphorylated at the indicated residue. Phospho-FAK (Tyr397) (D20B1) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR.

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK protein and a synthetic peptide corresponding to residues surrounding Ser893 of human FAK protein. Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr576/577 and Tyr925 of human FAK protein.

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).

  1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-13.
  2. Schaller, M.D. et al. (1994) Mol Cell Biol 14, 1680-8.
  3. Cobb, B.S. et al. (1994) Mol Cell Biol 14, 147-55.
  4. Chen, H.C. et al. (1996) J Biol Chem 271, 26329-34.
  5. Zhang, X. et al. (1999) Proc Natl Acad Sci U S A 96, 9021-6.
  6. Calalb, M.B. et al. (1995) Mol Cell Biol 15, 954-63.
  7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.
Entrez-Gene Id
5747
Swiss-Prot Acc.
Q05397
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 9330T

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Product # Size Price
9330T
1 Kit (4 x 20 µl) $ 310.0