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FAK Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

FAK Antibody Sampler Kit #9330

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FAK Antibody Sampler Kit: Image 1
Western blot analysis of extracts from various cell lines using FAK (D2R2E) Rabbit mAb.
FAK Antibody Sampler Kit: Image 2
Western blot analysis of extracts from Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr576/577) Antibody (upper) or FAK antibody (lower).
FAK Antibody Sampler Kit: Image 3
Western blot analysis of extracts from Jurkat cells, untreated or anti-CD3 antibody-treated (1 µg/ml for 10 minutes), using Phospho-FAK (Tyr925) Antibody (upper) or FAK antibody (lower).
FAK Antibody Sampler Kit: Image 4
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
FAK Antibody Sampler Kit: Image 5
Western blot analysis of extracts from A549 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-FAK (Tyr397) (D20B1) Rabbit mAb (upper) and FAK Antibody #3285 (lower).
FAK Antibody Sampler Kit: Image 6
Immunoprecipitation of FAK from U-87 MG cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or FAK (D2R2E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FAK (D2R2E) Rabbit mAb.
To Purchase # 9330
Cat. # Size Qty. Price
1 Kit  (4 x 20 microliters)
$ 380

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-FAK (Tyr576/577) Antibody 3281 20 µl
  • WB
  • IP
H M R 125 Rabbit 
Phospho-FAK (Tyr925) Antibody 3284 20 µl
  • WB
  • IP
H M 125 Rabbit 
Phospho-FAK (Tyr397) (D20B1) Rabbit mAb 8556 20 µl
  • WB
  • IP
H 125 Rabbit IgG
FAK (D2R2E) Rabbit mAb 13009 20 µl
  • WB
  • IP
H M 125 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 

Product Description

The FAK Antibody Sampler Kit provides an economical means of evaluating total FAK protein levels as well as FAK phosphorylated at specific sites. The kit contains enough primary and secondary antibody to perform two western blots with each antibody.

Specificity / Sensitivity

Each antibody in the FAK Antibody Sampler Kit detects endogenous levels of its target protein and does not typically cross react with other proteins. Activation state antibodies recognize target proteins only when phosphorylated at the indicated residue. Phospho-FAK (Tyr397) (D20B1) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK protein and a synthetic peptide corresponding to residues surrounding Ser893 of human FAK protein. Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr576/577 and Tyr925 of human FAK protein.


Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).


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Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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