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74437
Fanconi Anemia Antibody Sampler Kit

Fanconi Anemia Antibody Sampler Kit #74437

Western Blotting Image 1

Western blot analysis of extracts from various cell lines using FANCA (D1L2Z) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). As expected, GM06914 cells are negative for FANCA.

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Western Blotting Image 2

Western blot analysis of extracts from various cell types using FANCB (D9W6S) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts fro HeLa, U-2 OS, and GM16633 (PD20, FANCD2-negative) cells using FANCD2 (D5L5X) Rabbit mAb (upper) or α-Actinin (D6S6) Rabbit mAb #6487 (lower).

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Western Blotting Image 4

Western blot analysis of extracts from various cell lines using BRCA2 (D9S6V) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

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Western Blotting Image 5

Western blot analysis of extracts from SH-SY5Y, Saos-2 and Jurkat cells using BACH1/BRIP1 Antibody.

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Western Blotting Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IP Image 7

Immunoprecipitation of FANCA from 293T cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or FANCA (D1L2Z) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using FANCA (D1L2Z) Rabbit mAb.

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Western Blotting Image 8

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human FANCB (FANCB-Myc/DDK; +) protein, using FANCB (D9W6S) Rabbit mAb.

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Western Blotting Image 9

Western blot analysis of extracts from HeLa cells, untreated or treated with mitomycin C (2 μg/mL, 24 hr), using FANCD2 (D5L5X) Rabbit mAb. DNA damage caused by mitomycin C induces monoubiquitination of FANCD2, altering its electrophoretic mobility and increasing its apparent molecular weight (4).

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IP Image 10

Immunoprecipitation of FANCD2 from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is FANCD2 (D5L5X) Rabbit mAb. Western blot analysis was performed using FANCD2 (D5L5X) Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
FANCA (D1L2Z) Rabbit mAb 14657 20 µl
  • WB
  • IP
H 160 Rabbit IgG
FANCB (D9W6S) Rabbit mAb 14243 20 µl
  • WB
H M R 90 Rabbit IgG
FANCD2 (D5L5X) Rabbit mAb 16323 20 µl
  • WB
  • IP
H 155, 162 Rabbit IgG
BRCA2 (D9S6V) Rabbit mAb 10741 20 µl
  • WB
H 380 Rabbit IgG
BACH1/BRIP1 Antibody 4578 20 µl
  • WB
H 145 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Fanconi Anemia Antibody Sampler Kit provides an economical means of detecting members of the Fanconi Anemia signaling pathway. The kit includes enough antibody to perform two western blot experiments with each primary antibody.

Each antibody in the Fanconi Anemia Antibody Sampler kit detects endogenous levels of its target protein.

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to residues surrounding Ala514 of human FANCA, Pro280 of human FANCB, Gly995 of human FANCD2, and a recombinant protein specific to the carboxy terminus of human BRCA2. Polyclonal BACH1/BRIP1 Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to amino acids near the carboxy terminus of human BACH1. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.

Fanconi anemia (FA) is an autosomal recessive genetic disorder resulting in symptoms that include chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). In response to DNA damage, the FA nuclear complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCM) induces mono-ubiquitination of FANCD2 and FANCI (2).

Monoubiquitination of FANCD2 induces localization of FANCD2 to sites of DNA damage, where it interacts with BRCA1 (4). FANCJ/BRIP1, FANCD1/BRCA2, and FANCN/PALB2 are also recruited to sites of DNA damage. FA signaling is important in maintenance of chromosome stability and control of mitosis (3).

  1. Alter, B.P. (1996) Am J Hematol 53, 99-110.
  2. Fei, P. et al. (2005) Cell Cycle 4, 80-6.
  3. Nalepa, G. and Clapp, D.W. (2014) F1000Prime Rep 6, 23.
  4. Garcia-Higuera, I. et al. (2001) Mol Cell 7, 249-62.
Entrez-Gene Id
675 , 83990 , 2175 , 2187 , 2177
Swiss-Prot Acc.
P51587 , Q9BX63 , O15360 , Q8NB91 , Q9BXW9
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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