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8023
Fas (4C3) Mouse mAb
Primary Antibodies
Monoclonal Antibody

Fas (4C3) Mouse mAb #8023

Reviews ()
Citations (34)
Western Blotting Image 1: Fas (4C3) Mouse mAb

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with a human Fas construct, using Fas (4C3) Mouse mAb.

Western Blotting Image 2: Fas (4C3) Mouse mAb

Western blot analysis of extracts from various cell lines using Fas (4C3) Mouse mAb.

No image available
Immunofluorescence Image 1: Fas (4C3) Mouse mAb

Confocal immunofluorescent analysis of ACHN cells using Fas (4C3) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry Image 1: Fas (4C3) Mouse mAb

Flow cytometric analysis of fixed and permeabilized HT-1080 cells using Fas (4C3) Mouse mAb (blue) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red).

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:50
Flow Cytometry 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Fas (4C3) Mouse mAb recognizes endogenous levels of total Fas protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human Fas protein.

Background

Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9).

  1. Suda, T. et al. (1993) Cell 75, 1169-78.
  2. Lee, H.O. and Ferguson, T.A. (2003) Cytokine Growth Factor Rev 14, 325-35.
  3. Watanabe-Fukunaga, R. et al. (1992) Nature 356, 314-7.
  4. Hahne, M. et al. (1995) Int Immunol 7, 1381-6.
  5. Nagata, S. (1997) Cell 88, 355-65.
  6. Green, D.R. and Ferguson, T.A. (2001) Nat Rev Mol Cell Biol 2, 917-24.
  7. Walker, P.R. et al. (1997) J Immunol 158, 4521-4.
  8. Kayagaki, N. et al. (1995) J Exp Med 182, 1777-83.
  9. Tanaka, M. et al. (1995) EMBO J 14, 1129-35.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
To Purchase # 8023S
Product # Size Price
8023S
100 µl $ 268