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Fibrillarin (T298) Antibody #2147

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Inquiry Info. # 2147

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    Product Specifications

    REACTIVITY H M R Mk Dm B
    SENSITIVITY Endogenous
    MW (kDa) 37
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • Dm-D. melanogaster 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Fibrillarin (T298) Antibody detects endogenous levels of total fibrillarin protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, D. melanogaster, Bovine

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Thr298 of human fibrillarin. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place (1,2). Fibrillarin associates with several other structural proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence (3,4). The sequence, structure, and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development (5).

    Alternate Names

    34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; FBRL; FIB; FIB1; fibrillarin; FLRN; Histone-glutamine methyltransferase; Nop1; RNA, U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2'-O-methyltransferase fibrillarin; U6 snRNA 2'-O-methyltransferase fibrillarin

    For Research Use Only. Not for Use in Diagnostic Procedures.
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