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  3. Firefly Luciferase (E9W9V) Mouse Chimeric Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Firefly Luciferase (E9W9V) Mouse Chimeric Monoclonal Antibody #94817

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  • IF

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Mouse chimeric IgG2a
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Firefly Luciferase (E9W9V) Rabbit mAb #25039.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Firefly Luciferase (E9W9V) Mouse Chimeric Monoclonal Antibody recognizes exogenously expressed proteins containing firefly luciferase (P. pyralis).

    Species Reactivity:

    All Species Expected

    Source / Purification

    This recombinant chimeric antibody is engineered from Firefly Luciferase (E9W9V) Rabbit mAb #25039 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, Firefly Luciferase (E9W9V) Rabbit mAb #25039, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln230 of firefly luciferase (P. pyralis) protein.

    Background

    Luciferases are a group of enzymes that emit visible light in the presence of oxygen and luciferin. Firefly luciferase (FLuc) is a 62 kDa oxygenase isolated from the firefly, P. pyralis, that produces a yellow-green light with a peak emission wavelength of 550-570 nm (1). FLuc is widely used as a bioluminescent reporter, emitting light through an ATP-dependent reaction involving oxygen, D-luciferin, and a metal cation, typically magnesium (Mg2+) (2). FLuc is often genetically engineered into luciferase-containing plasmids as a reporter that can be measured by detection of firefly luciferase (P. pyralis) protein or its enzymatic activity (2,3).

    Alternate Names

    luciferin 4-monooxygenase

    Database Links

    UniProt ID: P08659


    Entrez-Gene Id: 116160065


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
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