Render Target: STATIC
Render Timestamp:
3/26/2025, 10:32:44 AM EDT
3/26/2025, 2:32:44 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:16:52.985
Product last modified at: 2025-01-01T09:04:45.234Z
Cell Signaling Technology Logo
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

FIZZ1 (E9O2P) Rabbit mAb #29744

Filter:
  • IF

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    FIZZ1 (E9O2P) Rabbit mAb recognizes endogenous levels of total mouse FIZZ1 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro42 of mouse FIZZ1 protein.

    Background

    Found in inflammatory zone 1 (FIZZ1) protein, also known as resistin-like molecule α (RELMα) and hypoxia-induced mitogenic factor (HIMF), is a secreted protein with a cysteine-rich carboxyl-terminus (1). FIZZ1 is a member of the RELM family of proteins which consists of four isoforms in mice and two isoforms in humans. The murine family members are FIZZ1, FIZZ2, FIZZ3, and FIZZ4 and the human proteins are Resistin and RELMβ (2). FIZZ1 is expressed in lung, bone marrow, and spleen tissue (1,3). FIZZ1 transcription is mediated by a T helper type 2 (Th2) cytokine environment and its upregulation has been observed in inflammatory and infectious environments, including allergic airway inflammation, colitis, and helminth infection (4,5). FIZZ1 has been shown to drive myeloid cell chemotaxis and to downregulate Th2 cytokine production, both in a Bruton’s tyrosine kinase (BTK)-dependent manner (6,7). FIZZ1 is induced in a mouse chronic hypoxia model of pulmonary hypertension and activates the PI3K/Akt pathway, driving proliferation of pulmonary microvascular smooth muscle cells (8). FIZZ1 has been identified as a marker for alternatively activated macrophages, also known as M2 macrophages, which are characterized as anti-inflammatory and are hallmarks of several disease states, including infection, allergy, diabetes, and cancer (7,9,10). 
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.