Western blot analysis of extracts from 293 cells, untransfected (-) or transfected with a Myc-tagged cDNA expression construct encoding human fumarase (hFumarase-Myc; +), using Fumarase (D9C5) Rabbit mAb (upper) and Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Western blot analysis of extracts from various cell lines using Fumarase (D9C5) Rabbit mAb.
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
Fumarase (D9C5) Rabbit mAb recognizes endogenous levels of total fumarase protein.
Human, Mouse, Rat, Hamster, Monkey
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly354 of human fumarase protein.
Fumarate hydratase (Fumarase, FH) is canonically regarded as a mitochondrial enzyme of the tricarboxylic acid cycle that catalyzes fumarate to malate (1,2). Studies have demonstrated that deficiencies in FH can lead to hereditary leiomyomatosis and renal cell cancer (1,3) or, in homozygous germline mutations, severe neurological and muscular disorders (1,2). There also exists a cytosolic fumarase isoenzyme that is involved in DNA damage repair (3).
Explore pathways + proteins related to this product.
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