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G-beta-L Antibody #3227

Filter:
  • WB
  • IHC

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 37
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    G-beta-L Antibody detects endogenous levels of total GβL protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln164 of human GβL. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).

    Alternate Names

    G protein beta subunit like; G protein beta subunit-like; Gable; GbetaL; GBL; LST8; Mammalian lethal with SEC13 protein 8; MGC111011; MLST8; MTOR associated protein, LST8 homolog; MTOR associated protein, LST8 homolog (S. cerevisiae); POP3; Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit LST8; WAT1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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