Render Target: STATIC
Render Timestamp: 2024-12-09T12:11:58.854Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-08-01 15:29:01.371
Product last modified at: 2024-12-06T21:30:10.001Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

G3BP1 (E9G1M) XP® Rabbit mAb #61559

Filter:
  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 68
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:2500 - 1:10000
    Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    G3BP1 (E9G1M) XP® Rabbit mAb recognizes endogenous levels of total G3BP1 protein. This antibody does not cross-react with G3BP2 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val218 of human G3BP1 protein.

    Background

    Ras GTPase-activating protein-binding protein 1, also known as GAP SH3 domain-binding protein 1 (G3BP1), was identified as a protein that interacts with the SH3 domain of Ras GTPase-activating protein (RasGap) (1). G3BP1 is involved in the regulation of multiple cellular processes, including mRNA decay and inhibition of translation initiation (2). Furthermore, G3BP1 is essential for the assembly of stress granules (SGs) and functions as an SG-nucleating protein (3). Research studies show that arginine demethylation of G3BP1 promotes SG assembly during oxidative stress (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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