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GAMT (F1A7Z) Rabbit Monoclonal Antibody #69029

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  • WB

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 26
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GAMT (F1A7Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total GAMT protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp130 of human GAMT protein.

    Background

    Guanidinoacetate N-methyltransferase (GAMT) is an enzyme member of the transferase protein family that functions in creatine synthesis. Creatine is a naturally occurring compound that plays a role in maintaining ATP levels within metabolically active tissues such as muscle and brain. While creatine is mainly synthesized in the kidneys and liver, it is also endogenously synthesized in the brain (1,2). Creatine synthesis occurs through the conversion of arginine and glycine to guanidinoacetate (GAA) by arginine:glycine amidinotransferase (AGAT). GAA is then methylated by GAMT, forming creatine (3). In the brain, this system is essential to maintaining the development and function of the central nervous system, with deficiency in either GAMT or AGAT leading to neurological consequences. GAMT deficiency is a disorder caused by mutations in the GAMT gene, which disrupts the creatine synthesis pathway, leading to developmental delays, varying intellectual disability, and seizure disorders (4,5).

    Alternate Names

    CCDS2; epididymis secretory protein Li 20; GAMT; Guanidinoacetate N-methyltransferase; HEL-S-20; PIG2; TP53I2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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