GAT3 Antibody #25438
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70, 140 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
GAT3 Antibody recognizes endogenous levels of total GAT3 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro604 of human GAT3 protein. Antibodies are purified by peptide affinity chromatography.
Background
The solute carrier 6 (SLC6) gene, also known as the neurotransmitter–sodium-symporter family or Na+/Cl- -dependent transporters, encodes for proteins that regulate neurotransmitter (NTT) transport, including monoamine transmitters serotonin, dopamine, and norepinephrine (SERT), GABA transmitters (GAT1, GAT2, GAT3, and BGT1), and glycine transmitters (GLYT1 and GLYT2) (1). The sodium- and chloride-dependent GABA transporter 3 (GAT3) is encoded by the solute carrier family 6 member 11 (SLC6A11) gene (2). GABA is the principal inhibitory neurotransmitter in the central nervous system and maintains the inhibitory tone that balances out neuronal excitation. This balance between neuronal excitation and inhibition is tightly regulated, in part by the GAT family of GABA transporters. These transporters terminate GABA inhibition by mediating cellular reuptake of GABA at the synaptic cleft. The human genome encodes four GABA transports (GAT-1-4). GAT-1 is found predominantly in the axon terminals of neurons whereas GAT-3 is expressed in astrocytes (3). Dysfunction of GABA transporters may contribute to diseases such as epilepsy as well as neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease (4-6).
- Kristensen, A.S. et al. (2011) Pharmacol Rev 63, 585-640.
- Soudijn, W. and van Wijngaarden, I. (2000) Curr Med Chem 7, 1063-79.
- Jin, X.T. et al. (2011) Front Syst Neurosci 5, 63.
- Sałat, K. and Kulig, K. (2011) Future Med Chem 3, 211-22.
- Chazalon, M. et al. (2018) Cell Rep 23, 1678-90.
- Fuhrer, T.E. et al. (2017) Neuroscience 351, 108-18.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.