Render Target: STATIC
Render Timestamp: 2024-10-14T09:59:53.471Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-30 01:59:02.614
Product last modified at: 2024-09-30T08:00:38.200Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

GATA-2 (E8B5C) Rabbit mAb #72445

Filter:
  • WB
  • IP
  • IHC
  • IF
  • ChIP
  • C&R
  • C&T

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 51
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • ChIP-Chromatin Immunoprecipitation 
    • C&R-CUT & RUN 
    • C&T-CUT & Tag 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    For optimal ChIP and ChIP-Seq results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.

    The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

    The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.
    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:2000 - 1:8000
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600
    Chromatin IP 1:50
    Chromatin IP-seq 1:50
    CUT&RUN 1:50
    CUT&Tag 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier free (BSA and azide free) version of this product see product #67509.

    Protocol

    Specificity / Sensitivity

    GATA-2 (E8B5C) Rabbit mAb recognizes endogenous levels of total GATA-2 protein. Non-specific staining was observed in squamous epithelium by immunohistochemistry.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly132 of human GATA-2 protein.

    Background

    The zinc finger transcription factor GATA-2 is widely expressed and plays an essential role in many developmental processes (1). Studies on GATA-2 knockout mice indicate that this protein is required in hematopoiesis (2). GATA-2 also inhibits the differentiation of white (3) and brown adipocytes (4) and has been shown to suppress the proliferation of neuronal progenitor cells (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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