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26168
PhosphoPlus ® GCN2 (Thr899) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus ® GCN2 (Thr899) Antibody Duet #26168

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PhosphoPlus ® GCN2 (Thr899) Antibody Duet: Image 1
Western blot analysis of extracts from various cell lines using GCN2 (E7G7E) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
PhosphoPlus ® GCN2 (Thr899) Antibody Duet: Image 2
Immunoprecipitation of GCN2 protein from HT-1376 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is GCN2 (E7G7E) Rabbit mAb. Western blot analysis was performed using GCN2 (E7G7E) Rabbit mAb.
PhosphoPlus ® GCN2 (Thr899) Antibody Duet: Image 3
Confocal immunofluorescent analysis of HT-1376 cells (left, positive) and BT-20 cells (right, negative) using GCN2 (E7G7E) Rabbit mAb (green). Blue = DAPI #4083 (fluorescent DNA dye).
PhosphoPlus ® GCN2 (Thr899) Antibody Duet: Image 4
Western blot analysis of extracts from HT-1376 cells, untreated (-) or treated with UV (50 mJ/cm2, 30 min recovery; +), using Phospho-GCN2 (Thr899) (E1V9M) Rabbit mAb (upper), GCN2 (E9H6C) Rabbit mAb #40457 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
PhosphoPlus ® GCN2 (Thr899) Antibody Duet: Image 5
Confocal immunofluorescent analysis of HT-1376 cells, untreated (left) or treated with UV (50 mJ/cm2, 30 min recovery; right), using Phospho-GCN2 (Thr899) (E1V9M) Rabbit mAb (green). Actin filaments were labeled with Alexa Fluor® 647 Phalloidin #8940 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).
PhosphoPlus ® GCN2 (Thr899) Antibody Duet: Image 6
Flow cytometric analysis of ACHN cells, untreated (blue) or treated with UV (100 mJ/cm2, 1 hr recovery; green), using Phospho-GCN2 (Thr899) (E1V9M) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
To Purchase # 26168S
Product # Size Price
26168S
1 Kit $ 529

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism of downregulating protein synthesis under a variety of stress conditions. Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), and hemin deficiency (HRI) can phosphorylate the eIF2 alpha subunit (1,2). GCN2 is also required for UV light-induced translation inhibition, and in vivo phosphorylation of murine GCN2 at Thr898 is induced by both UV irradiation and by leucine deprivation (3). UV-induced activation of NF-κB also requires GCN2, which may act simply by preventing translation of IκB-alpha to replace pools that have been ubiquitinated and degraded (4). Interestingly, proteasome inhibitors (MG132 and ALLN) activate the GCN2/eIF2alpha pathway, suggesting a pivotal role for this kinase in stress response and ubiquitin-mediated signaling (5). In vitro autophosphorylation of yeast GCN2 within its activation loop at Thr882 and Thr887 (Thr898 and Thr903 in mouse) has also been reported (6).
  1. Kaufman, R.J. (1999) Genes Dev 13, 1211-33.
  2. Sheikh, M.S. and Fornace, A.J. (1999) Oncogene 18, 6121-8.
  3. Deng, J. et al. (2002) Curr Biol 12, 1279-86.
  4. Jiang, H.Y. and Wek, R.C. (2005) Biochem J 385, 371-80.
  5. Jiang, H.Y. and Wek, R.C. (2005) J Biol Chem 280, 14189-202.
  6. Garcia-Barrio, M. et al. (2002) J Biol Chem 277, 30675-83.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.