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GEF-H1 Antibody #4145

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  • WB

Inquiry Info. # 4145

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    Product Specifications

    REACTIVITY H M R Mk B
    SENSITIVITY Endogenous
    MW (kDa) 120
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 
    • B-Bovine 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GEF-H1 Antibody recognizes endogenous levels of total GEF-H1 protein. In human and monkey cells, this antibody cross-reacts with an unrelated 70 kDa protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey, Bovine

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human GEF-H1. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Rho family small GTPases regulate processes such as cell migration, adhesion, proliferation, and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. GEF-H1 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (1). Loss of interaction between GEF-H1 and microtubules leads to activation of Rho (2). Phosphorylation of GEF-H1 at Ser886 (Ser885 in mouse), a site located in the 14-3-3 binding motif, has been implicated in recruitment of 14-3-3 and GEF-H1 to microtubules (3), and in the regulation of RhoA activity in response to mitotic kinases during cytokinesis (4).
    GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability (5,6). GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest (6).

    Alternate Names

    ARHG2; ARHGEF2; DKFZp547L106; DKFZp547P1516; FLJ45440; GEF; GEF-H1; GEFH1; Guanine nucleotide exchange factor H1; KIAA0651; Lfc; LFP40; Microtubule-regulated Rho-GEF; NEDMHM; P40; Proliferating cell nucleolar antigen p40; Rho guanine nucleotide exchange factor 2; Rho/Rac guanine nucleotide exchange factor (GEF) 2; Rho/Rac guanine nucleotide exchange factor 2; RhoGEF2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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