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94905
PhosphoPlus® Glycogen Synthase (Ser641) Antibody Duet

PhosphoPlus® Glycogen Synthase (Ser641) Antibody Duet #94905

Western Blotting Image 1

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or treated (+) with λ phosphatase and calf intestinal alkaline phosphatase (CIP), using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb (upper) or Glycogen Synthase (15B1) Rabbit mAb #3886 (lower).

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines, using Glycogen Synthase (15B1) Rabbit mAb.

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IHC-P (paraffin) Image 3

Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IHC-P (paraffin) Image 4

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Glycogen Synthase (15B1) Rabbit mAb.

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IHC-P (paraffin) Image 5

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb.

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IHC-P (paraffin) Image 6

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma, untreated (left) or λ phosphatase treated (right), using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb.

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IHC-P (paraffin) Image 7

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb.

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Glycogen Synthase (Ser641) (D4H1B) XP® Rabbit mAb 47043 100 µl
  • WB
  • IP
  • IHC
H M R Mk 85-90 Rabbit IgG
Glycogen Synthase (15B1) Rabbit mAb 3886 100 µl
  • WB
  • IP
  • IHC
H M R 84 Rabbit IgG

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver (1). Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals -- muscle isoform (GYS1) and liver isoform (GYS2) respectively (1). Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649 and Ser653) inhibiting its activity (2, 3). Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth (1).

  1. Favaro, E. et al. (2012) Cell Metab 16, 751-64.
  2. Mora, A. et al. (2005) FEBS Lett 579, 3632-8.
  3. Jensen, J. et al. (2012) Am J Physiol Endocrinol Metab 303, E82-9.
Entrez-Gene Id
2997
Swiss-Prot Acc.
P13807
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
CST is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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