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97537
Golgin-97 (CDF4) Mouse mAb
Primary Antibodies
Monoclonal Antibody

Golgin-97 (CDF4) Mouse mAb #97537

Citations (2)

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# Product Name Application Reactivity
  • WB
  • IP
  • IF
H M
No Current Image - Golgin-97 (CDF4) Mouse mAb

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 97
Source/Isotype Mouse 

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Golgin-97 (CDF4) Mouse mAb recognizes endogenous levels of total golgin-97 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human Golgin-97 protein.

Background

The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome (1). The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker. All four known mammalian GRIP domain-containing proteins (golgin-97, golgin-245, GCC88 and GCC185) are found in the TGN, share extensive alpha-helical structure, and form homodimers (2). While all four golgin proteins localize to the TGN, they exhibit different membrane-binding abilities and are found in distinct TGN regions (3). Golgin-97 and golgin-245 are targeted to the trans-Golgi network through an interaction between their GRIP domains and the Arl1 protein switch II region (4). Overexpression studies and siRNA assays with GRIP domain-containing proteins suggest that these proteins help to maintain trans-Golgi network integrity and function by controlling localization of TGN resident proteins (5). By using a Shiga toxin B fragment (STxB)-based in vitro transport assay and an E-cadherin transport model system, golgin-97 and its effector Arl1-GTP were shown to play a role in trans-Golgi endosomal trafficking (6,7). Research studies also suggest that golgin-97 may play a role in poxvirus morphogenesis and maturation (8,9).

  1. Griffith, K.J. et al. (1997) Arthritis Rheum 40, 1693-702.
  2. Luke, M.R. et al. (2005) Biochem J 388, 835-41.
  3. Derby, M.C. et al. (2004) J Cell Sci 117, 5865-74.
  4. Lu, L. and Hong, W. (2003) Mol Biol Cell 14, 3767-81.
  5. Yoshino, A. et al. (2003) J Cell Sci 116, 4441-54.
  6. Lu, L. et al. (2004) Mol Biol Cell 15, 4426-43.
  7. Lock, J.G. et al. (2005) Traffic 6, 1142-56.
  8. Alzhanova, D. and Hruby, D.E. (2006) J Virol 80, 11520-7.
  9. Alzhanova, D. and Hruby, D.E. (2007) Virology 362, 421-7.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
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