GP130 Antibody #3732
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 130 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
GP130 Antibody detects endogenous levels of total GP130 protein independent of phosphorylation.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human GP130. Antibodies are purified by protein A and peptide affinity chromatography.
Background
GP130 is a signal-transducing subunit shared by the receptors for the interleukin-6 (IL-6) family of cytokines (1-3). The binding of a ligand to its receptor induces the dimerization of GP130, leading to activation of the Jak tyrosine kinase and to tyrosine phosphorylation of GP130. These events lead to the activation of multiple signal-transduction pathways, such as the Stat, Ras-MAPK, and PI3 kinase pathways, whose activation is controlled by distinct regions of GP130 (4-7).
- Taga, T. (1996) J. Neurochem. 67, 1-10.
- Taga, T. (1997) Ann. Med. 29, 63-72.
- Taga, T. and Kishimoto, T. (1997) Annu. Rev. Immunol. 15, 797-819.
- Heinrich, P.C. et al. (1998) Biochem. J. 334, 297-314.
- Ihle, J.N. et al. (1994) Trends Biochem. Sci. 19, 222-227.
- Kurth, I. et al. (1999) J. Immunol. 162, 1480-1487.
- Paonessa, G. et al. (1995) EMBO J. 14, 1942-1951.
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