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XML generation date: 2026-05-20 02:54:45.056
Product last modified at: 2026-05-20T08:00:10.969Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Granzyme B (E5V2L) Feline Chimeric Monoclonal Antibody #28830

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  • IHC

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Feline chimeric IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Granzyme B (E5V2L) Rabbit Monoclonal Antibody #44153.

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Granzyme B (E5V2L) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total mouse Granzyme B protein. This antibody does not cross-react with human Granzyme B proteins. Nonspecific staining was observed in mouse kidney.

    Species Reactivity:

    Mouse

    Source / Purification

    This recombinant chimeric antibody is engineered from Granzyme B (E5V2L) Rabbit Monoclonal Antibody #44153 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, Granzyme B (E5V2L) Rabbit Monoclonal Antibody #44153, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala197 of mouse Granzyme B protein.

    Background

    Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3).

    Alternate Names

    AI553453; CCP; CCP-1/C1; CCP-1/C11; CCP1; Ctl; Ctla; CTLA-1; Ctla1; Cytotoxic cell protease 1; Fragmentin-2; GRAB; granzyme B; Granzyme B(G,H); GZB; Gzmb

    For Research Use Only. Not for Use in Diagnostic Procedures.
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