Render Target: STATIC
Render Timestamp: 2024-10-24T11:05:38.887Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-08-01 15:23:59.670
Product last modified at: 2024-10-07T13:30:11.238Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Gremlin Antibody #4383

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Transfected Only
    MW (kDa) 21
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Gremlin Antibody detects transfected levels of total gremlin protein.

    Species Reactivity:

    Mouse

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Human, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Val170 of human gremlin. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Gremlin is a secreted antagonist of bone morphogenetic proteins that play important roles during development. Gene targeting generates gremlin deficient mice that die shortly after birth due to absent kidneys and defects in lung septation (1-3). Gremlin also plays a role in cardiomyogenesis and angiogenesis (4,5). TGF-β can induce gremlin expression under pathological conditions. Gremlin colocalizes with TGF-β in tubulointerstitial fibrosis associated with chronic allograft nephropathy and is expressed in diabetic nephropathy in vivo (6-8). Gremlin is widely expressed in cancer associated stromal cells and can enhance cancer cell proliferation (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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