Grp75 (D13H4) XP® Rabbit mAb #3593
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #36890.
Protocol
Specificity / Sensitivity
Grp75 (D13H4) XP® Rabbit mAb detects endogenous levels of total Grp75 protein.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Grp75.
Background
Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).
- Kaul, S.C. et al. (1993) Biochem Biophys Res Commun 193, 348-55.
- Wadhwa, R. et al. (1993) J Biol Chem 268, 6615-21.
- Schneider, H.C. et al. (1994) Nature 371, 768-74.
- Wadhwa, R. et al. (1998) J Biol Chem 273, 29586-91.
- Wadhwa, R. et al. (2006) Int J Cancer 118, 2973-80.
- Kaul, S.C. et al. (2003) Exp Cell Res 286, 96-101.
Limited Uses
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