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9369
GSK-3 Antibody Sampler Kit
Primary Antibodies

GSK-3 Antibody Sampler Kit #9369

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Western blot analysis of extracts from various cell types using GSK-3α (D80E6) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma , untreated (left) or lambda phosphatase treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or PDGF-treated (green), using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb.

Western blot analysis of extracts from COS-7 cells, λ phosphatase- or PDGF-treated (100 μg/ml, 15 min), and PC-3 cells, untreated or λ phosphatase-treated, using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower).

Flow cytometric analysis of wild type mouse embryonic fibroblasts (MEFs) (green) and GSK-3β (-/-) MEFs (blue) using GSK-3β (D5C5Z) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody. (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right), using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

Confocal immunofluorescent analysis of wild type mouse embryonic fibroblasts (MEFs) (top row), GSK-3β (-/-) MEFs (middle row) , or PC-3 cells (bottom row), untreated (left), LY294002- and Wortmannin-treated (#9901 and #9951 respectively; center) or lambda phosphatase-treated (right), using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild type and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Western blot analysis of extracts from GSK-3α (-/-) and GSK-3β (-/-) mouse embryonic fibroblasts (MEF), untreated or insulin-treated (100 ng/ml, 20 min) , using Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Confocal immunofluorescent analysis of wild-type mouse embryonic fibroblasts (MEFs) (left), GSK-3α (-/-) MEFs (center)and GSK-3β (-/-) MEFs (right) using GSK-3β (D5C5Z) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild type, GSK-3α (-/-), and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb in the presence of control peptide (left) or Phospho-GSK-3α (Ser21) (36E9) Blocking Peptide #1027 (right).

Western blot analysis of extracts from GSK-3α (-/-) (lanes 1,2) and GSK-3β (-/-) (lanes 3,4) mouse embryonic fibroblast (MEF) cells, λ phosphatase or PDGF-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (upper) and α/β-Tubulin Antibody #2148 (lower). (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using GSK-3β (D5C5Z) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-GSK-3alpha (Ser 21) (36E9) Rabbit mAb.

Western blot analysis of extracts from PC-3 cells, untreated or LY294002/wortmannin-treated, using Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb (upper) or GSK-3β (27C10) Rabbit mAb #9315 (lower).

Immunohistochemical analysis of paraffin-embedded mouse pancreas using GSK-3β (D5C5Z) XP® Rabbit mAb.

Western blot analysis of extracts from COS-7 cells, λ-phosphatase or PDGF-treated, using Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb (upper) or GSK-3α Antibody #9338 (lower).

Immunohistochemical analysis of paraffin-embedded MEF cell pellets, wild type (left), GSK-3α (-/-) (middle) and GSK-3β (-/-) (right) using GSK-3β (D5C5Z) XP® Rabbit mAb. (MEF wild type, GSK-3β (-/-), and GSK-3α (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

Immunoprecipitation of GSK-3β from PC-12 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or GSK-3β (D5C5Z) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using GSK-3β (D5C5Z) XP® Rabbit mAb.

Western blot analysis of extracts from wild-type, GSK-3α (-/-), and GSK3β (-/-) mouse embryonic fibroblasts (MEFs) using GSK-3β (D5C5Z) XP® Rabbit mAb (upper) and GSK-3α/β (D75D3) XP® Rabbit mAb #5676 (lower). (MEF wild type, GSK-3α (-/-), and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada).

To Purchase # 9369T
Product # Size Price
9369T
1 Kit  (5 x 20 µl) $ 380

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
GSK-3α (D80E6) Rabbit mAb 4337 20 µl
  • WB
  • IP
H M R Hm Mk 51 Rabbit IgG
Phospho-GSK-3α (Ser21) (36E9) Rabbit mAb 9316 20 µl
  • WB
  • IHC
H M R Mk 51 Rabbit 
Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb 5558 20 µl
  • WB
  • IP
  • IF
  • F
H M R Hm 46 Rabbit IgG
Phospho-GSK-3α/β (Ser21/9) (D17D2) Rabbit mAb 8566 20 µl
  • WB
  • IP
H M R Hm Mk 46 GSK-3beta, 51 GSK-3alpha Rabbit IgG
GSK-3β (D5C5Z) XP® Rabbit mAb 12456 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R Mk 46 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The GSK-3 Antibody Sampler Kit contains primary and secondary antibodies to perform two Western blots with each antibody.

Specificity / Sensitivity

Each antibody in the GSK3 Antibody Sampler Kit recognizes only its specific target and does not cross-react with other family members. Phospho-GSK-3ß (Ser9) (D85E12) XP® Rabbit mAb reacts with denatured components of bovine serum, including BSA.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser9 of human GSK-3ß, residues around Ser21 of human GSK-3α, or to the sequence of human GSK-3α protein or by immunizing animals with recombinant protein specific to the carboxy terminus of human GSK-3ß protein.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

  1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
  2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
  3. Cross, D.A. et al. (1995) Nature 378, 785-9.
  4. Nusse, R. (1997) Cell 89, 321-3.
  5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.