Render Target: STATIC
Render Timestamp: 2024-10-09T10:17:14.780Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Polyclonal Antibody (Epitope Tag)
PDP - Template ID: *******04464df

GST-Tag Antibody #2622

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    GST-Tag Antibody detects transfected GST fusion proteins.

    Species Reactivity:

    All Species Expected

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a GST fusion protein. Antibodies are purified by protein A and GST affinity chromatography.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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