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R Recombinant
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Hepcidin (E8M5B) Rabbit mAb #29572

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  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 10
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Hepcidin (E8M5B) Rabbit mAb recognizes endogenous levels of total hepcidin protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln30 of human hepcidin protein.

    Background

    Hepcidin is a secreted peptide hormone that functions as a master regulator of systemic iron homeostasis (1). Hepcidin is synthesized as a preprohormone, primarily by hepatocytes; the preprohormone undergoes a series of cleavage events, culminating in the release of mature hepcidin into circulation, where it plays a critical role in regulating plasma iron levels (2). Hepcidin in circulation binds to ferroportin, a channel protein that regulates cellular iron export, and whose expression levels are post-translationally regulated via its interactions with hepcidin (3). Chronic overexpression of hepcidin leads to iron-restricted anemia in mammals, whereas hepcidin deficiency leads to disease states associated with iron overload. Hepcidin levels can thus serve as useful biomarkers for various disease states related to iron homeostasis (4-7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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