|8579T||1 Kit (9 x 20 microliters)||
|Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb 13619||20 µl||
||H M R||65-78||Rabbit IgG|
|LATS1 (C66B5) Rabbit mAb 3477||20 µl||
||H M Mk||140||Rabbit IgG|
|Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb 8699||20 µl||
||H M R Mk||24||Rabbit IgG|
|MOB1 (E1N9D) Rabbit mAb 13730||20 µl||
||H M R Hm Mk||24||Rabbit IgG|
|MST1 Antibody 3682||20 µl||
||H M R Mk B||59||Rabbit|
|MST2 Antibody 3952||20 µl||
||H M R Mk B||60||Rabbit|
|SAV1 (D6M6X) Rabbit mAb 13301||20 µl||
||H M||45||Rabbit IgG|
|Phospho-YAP (Ser127) (D9W2I) Rabbit mAb 13008||20 µl||
||H M R||65-78||Rabbit IgG|
|YAP/TAZ (D24E4) Rabbit mAb 8418||20 µl||
||H M Mk||55, 78||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Mst1 protein, or residues near the amino terminus of human Mst2 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human MOB1A protein, residues surrounding Gly180 of human LATS1 protein, residues near the carboxy terminus of human SAV1 protein, or residues surrounding Asp362 of human TAZ protein. Phospho-specific monoclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr35 of human MOB1 protein, residues surrounding Ser397 of human YAP protein isoform 1, or residues surrounding Ser127 of human YAP protein.
Hippo signaling is an evolutionarily conserved pathway that controls cell proliferation, apoptosis, and organ size in response to changing cell density levels (1,2). At relative low cell density, transcription co-activators YAP and TAZ bind transcription factors to induce expression of genes that favor cell growth and proliferation. As cell density increases, interaction between membrane-bound upstream hippo pathway regulators trigger activation of cytoplasmic kinases Mst1/2 and LATS1/2. Activated Mst kinase (the eponymous Hippo in Drosophila) associates with the adaptor Sav1 and phosphorylates MOB1 to activate LATS kinase, which phosphorylates YAP and TAZ to suppress cell proliferation (3).
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