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HLA-E (MEM-E/02) Mouse Monoclonal Antibody #73269

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 43
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:125 - 1:500
    Flow Cytometry (Fixed/Permeabilized) 1:1000 - 1:4000

    Storage

    Supplied in PBS (pH 7.4) containing 15 mM sodium azide. Store at 4°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HLA-E (MEM-E/02) Mouse Monoclonal Antibody recognizes endogenous levels of HLA-E protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant human HLA-E denatured heavy chain.

    Background

    HLA-E (HLA class I histocompatibility antigen, alpha chain E) is a non-classical MHC molecule that is expressed by B and T lymphocytes, natural killer (NK) cells, and macrophages, and is overexpressed in some tumors (1-3). Classical HLA class I molecules (HLA-A, -B, and -C) are highly polymorphic, affecting the peptide binding region (PBR). HLA-E has limited polymorphism that is restricted to two functional versions (3,5). HLA-E is a ligand of CD94/NKG2A, -B, and -C receptors, which are expressed on NK cells and T cells and play a role in the innate and adaptive immune system (3-5).

    Alternate Names

    DKFZp686P19218; EA1.2; EA2.1; HLA class I histocompatibility antigen, alpha chain E; HLA class I histocompatibility antigen, E alpha chain; HLA-6.2; HLA-E; HLAE; lymphocyte antigen; major histocompatibility complex, class I, E; MHC; MHC class I antigen E; MHC class Ib antigen; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; QA1; sHLA-E; Soluble HLA class I histocompatibility antigen, alpha chain E

    For Research Use Only. Not for Use in Diagnostic Procedures.
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