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HNF4α (G162) Antibody #3117

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Inquiry Info. # 3117

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    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 52
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    HNF4α (G162) Antibody detects endogenous levels of total HNF4α protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human HNF4α. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4α, in association with PGC-1α, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4α gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4α have been linked to maturity-onset diabetes of the young (MODY) (5).

    Alternate Names

    FLJ39654; FRTS4; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4-alpha; hepatocyte nuclear factor 4, alpha; HNF-4-alpha; HNF4; HNF4 alpha; HNF4A; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; HNF4alpha10/11/12; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF; TCF-14; TCF14; Transcription factor 14; Transcription factor HNF-4; transcription factor-14

    For Research Use Only. Not for Use in Diagnostic Procedures.
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