Western blot analysis of extracts from various cell lines using hnRNP L Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
|REACTIVITY||H Mk M R|
|MW (kDa)||60, 66|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
hnRNP L Antibody recognizes endogenous levels of total hnRNP L protein.
Human, Monkey, Mouse, Rat
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His313 of human hnRNP L protein. Antibodies are purified by protein A and peptide affinity chromatography.
Heterogeneous nuclear ribonucleoprotein L (hnRNP L) belongs to a family of RNA binding multiprotein complexes (hnRNP proteins) that facilitate pre-mRNA processing and transport of mRNA from the nucleus to cytoplasm (1-3). hnRNP L binds to CA repeat elements to regulate splicing and RNA stability (4-6). Binding of hnRNP L has been shown to occur in response to stress stimuli to regulate conformational change and subsequent translation of mRNAs (7). hnRNPL can promote apoptosis by enhancing translation of p53 mRNA (8) . In the context of tumor progression, hnRNPL has been shown to bind and modify translation of critical regulators of cell cycle and apoptosis (9,10).
Explore pathways + proteins related to this product.
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