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hnRNP Q/R (D18B2) Rabbit Monoclonal Antibody #8588

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 65-82
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    hnRNP Q/R (D18B2) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP Q and hnRNP R proteins.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys371 of human hnRNP Q protein.

    Background

    Heterogeneous nuclear ribonucleoprotein Q and R belong to a family of hnRNP proteins that are involved in RNA binding, RNA biosynthesis, and mRNA transport from the nucleus to the cytoplasm (1-3). These two proteins are encoded by different genes but have 83% homology. hnRNP Q has three alternative splice variants (hnRNP Q1-3) (1-3). Methylation of carboxy-terminal arginine residues is required for nuclear localization (4). hnRNP Q binds to AU-rich mRNA in conjunction with AUF1 and regulates mRNA decay (5). hnRNP Q isoforms play a crucial role in mediating nuclear function of survival of motor neuron (SMN) complex (6,7) and modulating RNA biosynthesis and hepatitis C virus replication (8). hnRNP R was identified recently and its function is still under investigation (9), however hnRNP R does not duplicate the biological function of hnRNP Q. Both hnRNP Q and R are present in cytoplasmic mRNP granules containing untranslated mRNAs (10) and both interact with SMN (6).

    Alternate Names

    dJ3J17.2; FLJ25714; FLJ31626; Glycine- and tyrosine-rich RNA-binding protein; GRY-RBP; GRYRBP; Heterogeneous nuclear ribonucleoprotein Q; Heterogeneous nuclear ribonucleoprotein R; hnRNP Q; hnRNP R; hnRNP-Q; hnRNP-R; HNRNPQ; HNRNPR; HNRPQ; HNRPQ1; HNRPR; NS1-associated protein 1; NSAP1; PP68; synaptotagmin binding cytoplasmic RNA interacting protein; synaptotagmin binding, cytoplasmic RNA interacting protein; Synaptotagmin-binding, cytoplasmic RNA-interacting protein; SYNCRIP

    For Research Use Only. Not for Use in Diagnostic Procedures.
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