HS1 (D5A9) XP® Rabbit mAb #3892
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:100 |
| Immunofluorescence (Frozen) | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #63068.
Protocol
Specificity / Sensitivity
HS1 (D5A9) XP® Rabbit mAb detects endogenous levels of total HS1 protein. This antibody does not recognize human HS1 protein. HS1 has a calculated size of 54 kDa, but has an apparent molecular weight of 80 kDa on SDS-PAGE gels.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu310 of mouse HS1.
Background
HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).
- Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
- Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
- Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
- Hata, D. et al. (1994) Immunol Lett 40, 65-71.
- Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
- Gomez, T.S. et al. (2006) Immunity 24, 741-52.
- Kahner, B.N. et al. (2007) Blood 110, 2449-56.
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