HSP60 (D307) Antibody #4870
- WB
- IF
- F
Supporting Data
| REACTIVITY | H M R Mk Dm |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Dm-D. melanogaster
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
HSP60 (D307) Antibody detects endogenous levels of total HSP60 protein. This antibody does not cross-react with other HSPs.
Species Reactivity:
Human, Mouse, Rat, Monkey, D. melanogaster
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Asp307 of human HSP60. Antibodies are purified by protein A and peptide affinity chromatography.
Background
In both prokaryotic and eukaryotic cells the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones (1-3). HSP60 has primarily been known as a mitochondrial protein that is important for folding key proteins after import into the mitochondria (4). Research studies have shown that a significant amount of HSP60 is also present in the cytosol of many cells, and that it is induced by stress, inflammatory and immune responses, and autoantibodies correlated with Alzheimer's, coronary artery diseases, MS, and diabetes (5-8).
Limited Uses
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