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9770
IAP Family Antibody Sampler Kit

IAP Family Antibody Sampler Kit #9770

Western Blotting Image 1

Western blot analysis of extracts from various cell lines using c-IAP1 (D5G9) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from Raji, Ramos and SR cell lines, using c-IAP2 (58C7) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of lysates from Raji (human), BaF3 (mouse) and C6 (rat) cell lines using Survivin (71G4B7E) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from HeLa, Raji and COS cell lines, using XIAP (3B6) Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines using Livin (D61D1) XP® Rabbit mAb.

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Western Blotting Image 6

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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Western Blotting Image 7

Western blot analysis of extracts from HeLa cells, mock transfected (-) or transfected with human c-IAP1 (+), using c-IAP1 (D5G9) Rabbit mAb.

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Western Blotting Image 8

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® Survivin siRNA II (+), using Survivin (71G4B7E) Rabbit mAb #2808 and α-Tubulin (11H10) Rabbit mAb #2125. Survivin (71G4B7E) Rabbit mAb confirms silencing of survivin expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of survivin siRNA.

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IF-IC Image 9

Confocal immunofluorescent analysis of SK-MEL-28 cells (left) and HeLa cells (right) using Livin (D61D1) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Survivin (71G4B7E) Rabbit mAb.

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IHC-P (paraffin) Image 11

Immunohistochemical analysis of paraffin-embedded human lung carcinoma showing nuclear localization using Survivin (71G4B7E) Rabbit mAb #2808.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Survivin (71G4B7E) Rabbit mAb in the presence of control peptide (left) or Survivin Blocking Peptide #1037 (right).

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human pituitary adenoma using Survivin (71G4B7E) Rabbit mAb.

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IHC-F (frozen) Image 14

Immunohistochemical analysis of frozen H1650 xenograft showing nuclear localization using Survivin (71G4B7E) Rabbit mAb.

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Flow Cytometry Image 15

Flow cytometric analysis of untreated Jurkat cells using Survivin (71G4B7E) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

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IF-IC Image 16

Confocal immunofluorescent analysis of HeLa cells using Survivin (71G4B7E) Rabbit mAb (green). Mitochondria have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
c-IAP1 (D5G9) Rabbit mAb 7065 20 µl
  • WB
  • IP
H 62 Rabbit IgG
c-IAP2 (58C7) Rabbit mAb 3130 20 µl
  • WB
  • IP
H 70 Rabbit IgG
Survivin (71G4B7) Rabbit mAb 2808 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 16 Rabbit IgG
XIAP (3B6) Rabbit mAb 2045 20 µl
  • WB
H Mk 53 Rabbit IgG
Livin (D61D1) XP® Rabbit mAb 5471 20 µl
  • WB
  • IP
  • IF
H 34, 36 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The IAP Family Antibody Sampler Kit provides an economical means to investigate the expression of various IAP family members within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Each antibody in the IAP Family Antibody Sampler Kit detects endogenous levels of its respective target.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu28 of human c-IAP1 protein. The antibody is purified by protein A and affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Val117 of human c-IAP2, Cys60 of human Survivin, Ser245 of human XIAP, and Ala195 of human Livin.

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain (1,2). Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression (3-5). In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9 (5,6). In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase (2).

  1. Deveraux, Q.L. and Reed, J.C. (1999) Genes Dev 13, 239-52.
  2. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
  3. Altieri, D.C. et al. (1999) Lab Invest 79, 1327-33.
  4. Tamm, I. et al. (2000) Clin Cancer Res 6, 1796-803.
  5. Kasof, G.M. and Gomes, B.C. (2001) J Biol Chem 276, 3238-46.
  6. Deveraux, Q.L. et al. (1997) Nature 388, 300-4.
Entrez-Gene Id
329 , 330 , 79444 , 332 , 331
Swiss-Prot Acc.
Q13490 , Q13489 , Q96CA5 , O15392 , P98170
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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To Purchase # 9770T

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Product # Size Price
9770T
1 Kit (5 x 20 µl) $ 372.0