IκB-ζ Antibody #9244
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 85 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
IκB-ζ (MAIL, INAP) is a unique IκB family member homologous to Bcl-3 and induced by IL-1 and Toll-like receptor (TLR) ligands (9-11). Like other family members, it contains carboxyl terminal ankyrin-repeats responsible for interaction with NF-κB, particularly p50. Unlike classical IκB family members (α, β, ε) which inhibit NF-κB translocation and are rapidly degraded upon cytokine treatment, IκB-ζ is cytokine-inducible and localized to the nucleus where it regulates NF-κB DNA binding and transactivation (12-14). Induction of IκB-ζ is required for TLR/IL-1 induction of a subset of NF-κB target genes, including IL-6 (15). However, the IκB-ζ can also inhibit transactivation of other targets, such as TNF-α (14,15).
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- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
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- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
- Yamazaki, S. et al. (2001) J Biol Chem 276, 27657-62.
- Kitamura, H. et al. (2000) FEBS Lett 485, 53-6.
- Haruta, H. et al. (2001) J Biol Chem 276, 12485-8.
- Matsuo, S. et al. (2007) Biochem J 405, 605-15.
- Totzke, G. et al. (2006) J Biol Chem 281, 12645-54.
- Motoyama, M. et al. (2005) J Biol Chem 280, 7444-51.
- Yamamoto, M. et al. (2004) Nature 430, 218-22.
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