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  3. IL-1 alpha Antibody

IL-1 alpha Antibody #84618

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 30
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IL-1 alpha Antibody recognizes endogenous levels of total IL-1α protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human IL-1α protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Interleukin 1 alpha (IL-1α) belongs to the IL-1 family of cytokines with 11 members including IL-1β. IL-1α is expressed in many cell types of both hematopoietic and non-hematopoietic origins under steady state, and its expression can be increased in response to appropriate stimuli (1,2). Like IL-1β, IL-1α is also synthesized as a precursor (pro-IL-1α) and can be cleaved into smaller mature forms. However, both pro-IL-1α and the cleaved form of IL-1α are biologically active and can activate the signaling pathway through the membrane receptor IL-1R1. IL-1α is active both as a secreted form and as a membrane-bound form. Due to such characteristics, passive leakage of IL-1α from dying cells can activate inflammation, leading some researchers to consider IL-1α as a key “alarmin in the cell” that alerts the host to damage or injury (3,4). In addition, IL-1α can also enter the nucleus to modulate transcription (5,6).

    Alternate Names

    Hematopoietin-1; IL-1 alpha; IL-1A; IL1; IL1-ALPHA; IL1A; IL1F1; interleukin 1 alpha; interleukin 1, alpha; Interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha

    Database Links

    UniProt ID: P01583


    Entrez-Gene Id: 3552


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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