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PhosphoSitePlus® Resource

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
NLRP3 (D4D8T) Rabbit mAb 15101 20 µl
Western Blotting Immunoprecipitation
H M 110 Rabbit IgG
AIM2 (D5X7K) Rabbit mAb 12948 20 µl
Western Blotting Immunoprecipitation
H 40 Rabbit IgG
NLRC4 (D5Y8E) Rabbit mAb 12421 20 µl
Western Blotting Immunoprecipitation
H 110 Rabbit IgG
Caspase-1 (D7F10) Rabbit mAb 3866 20 µl
Western Blotting Immunoprecipitation
H 48, 20 Rabbit IgG
NALP1 Antibody 4990 20 µl
Western Blotting
H M R 165, 70 Rabbit 
TMS1 (E1E3I) Rabbit mAb 13833 20 µl
Western Blotting Immunoprecipitation
H 22, 19, 15 Rabbit IgG
Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb 4199 20 µl
Western Blotting Immunoprecipitation
H 20, 22 Rabbit IgG
Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb 83186 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H 17 Rabbit IgG
IL-1β (D3U3E) Rabbit mAb 12703 20 µl
Western Blotting Immunofluorescence Flow Cytometry
H 17, 31 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Inflammasome Antibody Sampler Kit provides an economical means of detecting multiple inflammasome components. The kit contains enough primary antibodies to perform at least two western blot experiments.


Specificity / Sensitivity

Each antibody in the Inflammasome Antibody Sampler Kit detects endogenous levels of its target protein. AIM2 (D5X7K) Rabbit mAb detects a 22 kDa band of unknown origin in some cell lines. Caspase-1 (D7F10) Rabbit mAb detects endogenous levels of full-length human Caspase-1; the activated p20 subunit was detected by over-expression. NALP1 Antibody detects endogenous levels of total NALP1 protein and also detects a 70 kDa protein that correlates with a predicted short form (NALP1s) that lacks the leucine repeat region. TMS1 (E1E3I) Rabbit mAb can detect three known isoforms of TMS1. Cleaved Caspase-1 (Asp297) (D57A2) Rabbit mAb detects endogenous levels of the p20 subunit of human caspase-1 only upon cleavage at Asp297. Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb recognizes endogenous levels of mature IL-1β protein only when cleaved at Asp116. IL-1β (D3U3E) Rabbit mAb is not able to detect endogenous levels of mature IL-1β. It can detect up to 100 pg of recombinant mature IL-1β.


Source / Purification

Monoclonal and polyclonal antibodies are produced by immunizing animals with recombinant human IL-1β protein or with synthetic peptides corresponding to residues adjacent to Asp297 human caspase-1, residues adjacent to Asp116 of human IL-1β, residues surrounding Ala306 of mouse NLRP3, Lys93 of human AIM2, Leu942 of human NLRC4, Gly1081 of human NALP1, and the carboxy terminus of human TMS1 isoform 1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

The innate immune system works as the first line of defense in protection from pathogenic microbes and host-derived signals of cellular distress. One way in which these “danger” signals trigger inflammation is through activation of inflammasomes, which are multiprotein complexes that assemble in the cytosol after exposure to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) and result in the activation of caspase-1 and subsequent cleavage of proinflammatory cytokines IL-1β and IL-18 (Reviewd in 1-6). Inflammasome complexes typically consist of a cytosolic pattern recognition receptor (PRR; a nucleotide-binding domain and leucine-rich-repeat [NLR] or AIM2-like receptor [ALR] family member), an adaptor protein (ASC/TMS1), and pro-caspase-1. A number of distinct inflammasome complexes have been identified, each with a unique PRR and activation triggers. The best characterized is the NLRP3 complex, which contains NLRP3, ASC, and pro-caspase-1. The NLRP3 inflammasome is activated in a two-step process. First, NF-κB signaling is induced through PAMP- or DAMP-mediated activation of TLR4 or TNFR, resulting in increased expression of NLRP3, pro-IL-1β, and pro-IL-18 (priming step, signal 1). Next, indirect activation of NLRP3 occurs by a multitude of signals (whole pathogens, PAMPs/DAMPs, potassium efflux, lysosomal-damaging environmental factors [uric acid, silica, alum] and endogenous factors [amyloid-β, cholesterol crystals], and mitochondrial damage), leading to complex assembly and activation of caspase-1 (signal 2). The complex inflammasome structure is built via domain interactions among the protein components. Other inflammasomes are activated by more direct means: double-stranded DNA activates the AIM2 complex, anthrax toxin activates NLRP1, and bacterial flagellin activates NLRC4. Activated caspase-1 induces secretion of proinflammatory cytokines IL-1β and -18, but also regulates metabolic enzyme expression, phagosome maturation, vasodilation, and pyroptosis, an inflammatory programmed cell death. Inflammasome signaling contributes to the onset of a number of diseases, including atherosclerosis, type II diabetes, Alzheimer’s disease, and autoimmune disorders.


1.  Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20.

2.  Guo, H. et al. (2015) Nat Med 21, 677-87.

3.  Jo, E.K. et al. (2016) Cell Mol Immunol 13, 148-59.

4.  Rathinam, V.A. and Fitzgerald, K.A. (2016) Cell 165, 792-800.

5.  Shao, B.Z. et al. (2015) Front Pharmacol 6, 262.

6.  Schroder, K. and Tschopp, J. (2010) Cell 140, 821-32.


Entrez-Gene Id 9447 , 29108 , 834 , 3553 , 22861 , 58484 , 216799
Swiss-Prot Acc. O14862 , Q9ULZ3 , P29466 , P01584 , Q9C000 , Q9NPP4 , Q8R4B8


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

32961
Inflammasome Antibody Sampler Kit