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IRG1 (D6H2Y) Rabbit Monoclonal Antibody #77510

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  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 53
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IRG1 (D6H2Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRG1 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly460 of human IRG1 protein.

    Background

    IRG1 (Immune-responsive gene 1) is one of the most up-regulated genes in macrophages under proinflammatory conditions (1). It is also highly expressed in the pregnant uterus during implantation (2,3). IRG1 is a cis-aconitate decarboxylase that produces itaconic acid by decarboxylating cis-aconitic acid, an intermediate of the tricarboxylic acid cycle (4). Itaconic acid is an endogenous inhibitor of succinate dehydrogenase, linking macrophage metabolic rewiring and regulation of inflammation (5,6).

    Alternate Names

    ACOD1; Aconitate decarboxylase; Aconitate decarboxylase 1; CAD; Cis-aconitate decarboxylase; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; Immune-responsive gene 1 protein homolog; immunoresponsive 1 homolog; immunoresponsive 1 homolog (mouse); IRG1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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