Render Target: STATIC
Render Timestamp: 2024-11-08T11:06:02.939Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:24:41.164
Product last modified at: 2024-10-10T11:45:16.641Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

IWS1 Antibody #5681

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 140
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    IWS1 Antibody detects endogenous levels of total IWS1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IWS1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Various steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation (1,2). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps (1,2). IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone (1,2). IWS1 also recruits another CTD-binding protein, HYPB/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation (2). Thus, IWS1 links Spt6 and HYPB/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level (2).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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