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8224
PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet

PhosphoPlus® Jak2 (Tyr1007/Tyr1008) Antibody Duet #8224

Western Blotting Image 1

Western blot analysis of extracts from UT-7 and BaF3 cells, untreated or treated with GM-CSF or IL-3 (5 minutes), using Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb #3776 (top) or total Jak2 (D2E12) XP® Rabbit mAb #3230 (bottom).

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Western Blotting Image 2

Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.

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Western Blotting Image 3

Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP® Rabbit mAb.

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IHC-P (paraffin) Image 4

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mAb.

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IHC-P (paraffin) Image 5

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).

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Flow Cytometry Image 6

Flow cytometric analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

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IF-IC Image 7

Confocal immunofluorescent analysis of K-562 cells using Jak2 (D2E12) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Jak2 (Tyr1007/1008) (C80C3) Rabbit mAb 3776 100 µl
  • WB
H M R 125 Rabbit IgG
Jak2 (D2E12) XP® Rabbit mAb 3230 100 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H M R 125 Rabbit IgG

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-5.
  3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
  4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
  5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
Entrez-Gene Id
3717
Swiss-Prot Acc.
O60674
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
Select antibodies in this kit are sold under a license from Chemicon International, Inc. relating to U.S. Patent No. 5,658,791.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

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