Interested in promotions? | Click here >>
25497
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

JARID1/KDM5 Histone Demethylase Antibody Sampler Kit #25497

Reviews ()
Citations (0)
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 1
Western blot analysis of extracts from MCF7, NCCIT, and COS-7 cell lines using Jarid1B (E2X6N) Rabbit mAb.
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 2
Western blot analysis of extracts from various cell lines using JARID1A (D28B10) Rabbit mAb.
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 3
Western blot analysis of extracts from NCCIT and F9 cell lines using JARID1C (D29B9) Rabbit mAb.
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 4
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 5
Western blot analysis of extracts from various cell lines using JARID1D (E4D4B) Rabbit mAb.
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 6
Immunoprecipitation of Jarid1B from MCF7 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Jarid1B (E2X6N) Rabbit mAb. Western blot analysis was performed using Jarid1B (E2X6N) Rabbit mAb.
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 7
Immunoprecipitation of JARID1D protein from THP-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is JARID1D (E4D4B) Rabbit mAb. Western blot analysis was performed using JARID1D (E4D4B) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 8
Chromatin immunoprecipitations were performed with cross-linked chromatin from K562 cells and Jarid1B (E2X6N) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across GAPDH, a known target gene of Jarid1B (see additional figure containing ChIP-qPCR data).
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 9
Chromatin immunoprecipitations were performed with cross-linked chromatin from K562 cells and Jarid1B (E2X6N) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across chromosome 12 (upper), including GAPDH (lower), a known target gene of Jarid1B (see additional figure containing ChIP-qPCR data).
JARID1/KDM5 Histone Demethylase Antibody Sampler Kit: Image 10
Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and either Jarid1B (E2X6N) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516,

SimpleChIP® Human γ-Actin Intron 3 Primers #5047, SimpleChIP® Human DHFR Intron 1 Primers #7531, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

To Purchase # 25497
Cat. # Size Qty. Price
25497T
1 Kit  (4 x 20 microliters) $ 336

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
JARID1A (D28B10) Rabbit mAb 3876 20 µl
  • WB
  • IP
H M 200 Rabbit IgG
JARID1B (E2X6N) Rabbit mAb 15327 20 µl
  • WB
  • IP
  • ChIP
H M Mk 180 Rabbit IgG
JARID1C (D29B9) Rabbit mAb 5361 20 µl
  • WB
  • IP
H M 180 Rabbit IgG
JARID1D (E4D4B) Rabbit mAb 78995 20 µl
  • WB
  • IP
H Mk 190 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The JARID1/KDM5 Histone Demethylase Antibody Sampler Kit provides an economical means for detecting the JARID1/KDM5 family of proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Each antibody in the JARID1/KDM5 Histone Demethylase Antibody Sampler Kit detects endogenous levels of its target protein. JARID1A (D28B10) Rabbit mAb detects endogenous levels of both isoforms of total JARID1A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala274 of human JARID1A protein and Leu830 of human JARID1C protein, near the amino terminus of human JARID1B protein, and near the carboxy terminus of human JARID1D protein.

Background

The methylation state of lysine residues in histone proteins is a major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families (3). The JARID (Jumonji/AT-rich interactive domain-containing protein) family contains four members: JARID1A (also RBP2 and RBBP2), JARID1B (also PLU-1), JARID1C (also SMCX), and JARID1D (also SMCY) (4). In addition to the JmJC domain, these proteins contain JmJN, BRIGHT, C5HC2 zinc-finger, and PHD domains, the latter of which binds to methylated histone H3 (Lys9) (4). All four JARID proteins demethylate di- and tri-methyl histone H3 Lys4; JARID1B also demethylates mono-methyl histone H3 Lys4 (5-7). JARID1A is a critical RB-interacting protein and is required for Polycomb-Repressive Complex 2 (PRC2)-mediated transcriptional repression during ES cell differentiation (8). A JARID1A-NUP98 gene fusion is associated with myeloid leukemia (9). JARID1B, which interacts with many proteins including c-Myc and HDAC4, may play a role in cell fate decisions by blocking terminal differentiation (10-12). JARID1B is overexpressed in many breast cancers and may act by repressing multiple tumor suppressor genes, including BRCA1 and HOXA5 (13,14). JARID1C has been found in a complex with HDAC1, HDAC2, G9a, and REST, which binds to and represses REST target genes in non-neuronal cells (7). JARID1C mutations are associated with X-linked mental retardation and epilepsy (15,16). JARID1D is uniquely localized to the Y chromosome, and functions as a tumor suppressor by repressing genes associated with cell invasiveness (17). JARID1D is frequently mutated in metastatic prostate tumors, and low JARID1D levels are associated with poor prognosis in prostate cancer patients (17).
  1. Kubicek, S. et al. (2006) Ernst Schering Res Found Workshop, 1-27.
  2. Lin, W. and Dent, S.Y. (2006) Curr Opin Genet Dev 16, 137-42.
  3. Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27.
  4. Benevolenskaya, E.V. (2007) Biochem Cell Biol 85, 435-43.
  5. Christensen, J. et al. (2007) Cell 128, 1063-76.
  6. Yamane, K. et al. (2007) Mol Cell 25, 801-12.
  7. Tahiliani, M. et al. (2007) Nature 447, 601-5.
  8. Pasini, D. et al. (2008) Genes Dev 22, 1345-55.
  9. van Zutven, L.J. et al. (2006) Genes Chromosomes Cancer 45, 437-46.
  10. Secombe, J. et al. (2007) Genes Dev 21, 537-51.
  11. Barrett, A. et al. (2007) Int J Cancer 121, 265-75.
  12. Dey, B.K. et al. (2008) Mol Cell Biol 28, 5312-27.
  13. Barrett, A. et al. (2002) Int J Cancer 101, 581-8.
  14. Lu, P.J. et al. (1999) J Biol Chem 274, 15633-45.
  15. Tzschach, A. et al. (2006) Hum Mutat 27, 389.
  16. Jensen, L.R. et al. (2005) Am J Hum Genet 76, 227-36.
  17. Li, N. et al. (2016) Cancer Res 76, 831-43.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.