Render Target: STATIC
Render Timestamp: 2024-12-13T11:13:17.722Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-05-10 22:31:19.190
Product last modified at: 2024-11-27T13:00:28.891Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Ki-67 (8D5) Mouse mAb #9449

Filter:
  • IHC
  • IF
  • F

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:800 - 1:3200
    Immunofluorescence (Immunocytochemistry) 1:1600 - 1:6400
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    For a carrier-free (BSA and azide free) version of this product see product #62548.

    Protocol

    Specificity / Sensitivity

    Ki-67 (8D5) Mouse mAb recognizes endogenous levels of total Ki-67 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Ki-67 protein.

    Background

    Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein (1) that is universally expressed among proliferating cells and absent in quiescent cells (2). Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival (3). Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases (4).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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