Render Target: STATIC
Render Timestamp: 2024-11-08T10:24:31.747Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-08-01 15:27:14.434
Product last modified at: 2024-10-17T12:15:13.258Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

LAMC1 Antibody #92921

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 220-250
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LAMC1 antibody recognizes endogenous levels of total LAMC1 protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1365 of human LAMC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Laminins are a family of proteins important for maintaining cellular basement membrane (BM) structure and function (1). Laminins exist as heterotrimers of alpha (LAMA), beta (LAMB), and gamma (LAMC) chains. LAMC1 (laminin gamma-1) is a ubiquitously expressed gamma chain, and has three distinct functional domain structures. The N-terminal LN domain binds to specific alpha and beta chains to form a unique laminin heterotrimer. The LE domain mediates interactions with nidogens, while the C-terminal coil-coil fragment is necessary for agrin association. Collectively, these interactions are critical for stabilization and function of the BM (1). Genetic ablation of LAMC1 has demonstrated a critical role for this protein in embryo implantation, lung and kidney development, and neuronal Schwann cell myelination and regeneration, in addition to Trypanosome infection (2-5). Upregulation of LAMC1 has also been associated with tumor progression in multiple tumor types (6-9), possibly by creating a BM environment that is favorable for cancer cell metastasis and invasion.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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