Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

LAT1/SLC7A5 (E9O4D) Rabbit Monoclonal Antibody #32683

Filter:
  • WB
  • IP
  • IF

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 39
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LAT1/SLC7A5 (E9O4D) Rabbit monoclonal antibody recognizes endogenous levels of total LAT1/SLC7A5 protein.

    LAT1/SLC7A5 (E9O4D) Rabbit monoclonal antibody lacks sensitivity in some low-expressing cell lines by immunofluorescence.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LAT1/SLC7A5 protein.

    Background

    L-type amino acid transporter 1 (LAT1), also known as solute carrier family 7 member 5 (SLC7A5), is a high-affinity neutral transporter of larger amino acids. It facilitates cellular amino acid uptake in a sodium-independent manner (1-2) and selectively transports D- and L-isomers of small neutral amino acids (3). LAT1 also regulates amino acid exchange in conjunction with SLC1A5 (2,4-6). Transport of thyroid hormones across the placenta is established via LAT1 during normal fetal development (7). LAT1 promotes neuronal cell proliferation by regulating amino acid transport across the blood-brain barrier (8). LAT1 is upregulated in various cancer types, including breast cancer, lung cancer, prostate cancer, and gliomas (9,10). High expression of LAT1 is detected in non-small cell lung cancer with lymph node metastases (9,11,12). Increased LAT1 expression is a novel biomarker of high-grade malignancy in prostate cancers (12). Inhibition of LAT1 suppresses tumor cell growth in several tumor types (10,13).

    Alternate Names

    4F2 LC; 4F2 light chain; 4F2LC; CD98; CD98 light chain; CD98LC; D16S469E; E16; hLAT1; Integral membrane protein E16; L-type amino acid transporter 1; large neutral amino acids transporter 1; Large neutral amino acids transporter small subunit 1; LAT1; MPE16; SLC7A5; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (amino acid transporter light chain, L system), member 5; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5; Solute carrier family 7 member 5; y+ system cationic amino acid transporter

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.