Buy 3 Get a 4th Free* | Learn More >>
5132
PhosphoPlus® LKB1 (Ser428) Antibody Duet

PhosphoPlus® LKB1 (Ser428) Antibody Duet #5132

Western Blotting Image 1

Western blot analysis of extracts from various cell lines, untreated or treated with TPA #9905 and UV, using Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb (upper) and LKB1 (D60C5) Rabbit mAb #3047 (lower).

Learn more about how we get our images
Western Blotting Image 2

Western blot analysis of extracts from various cell lines using LKB1 (D60C5) Rabbit mAb.

Learn more about how we get our images
Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-LKB1 (Ser428) (C67A3) Rabbit mAb 3482 100 µl
  • WB
H M R Mk 54 Rabbit 
LKB1 (D60C5) Rabbit mAb 3047 100 µl
  • WB
H M R Mk 54 Rabbit IgG

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).

Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

  1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
  2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
  3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
  4. Hardie, D.G. (2004) J. Cell Sci. 117, 5479-5487.
  5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
  6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
  7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.
Entrez-Gene Id
6794
Swiss-Prot Acc.
Q15831
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

Upstream / Downstream

pathwayImage

Explore pathways related to this product.

To Purchase # 5132S

Product Number Size Price
5132S 1 Kit (10 western blots) $472.00.0
Quantity Subtotal
$0.00