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MAG (D4G3) Horse Chimeric Monoclonal Antibody

MAG (D4G3) Horse Chimeric Monoclonal Antibody #98420

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Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)
Source/Isotype	Horse chimeric IgG4

Application Key:

IF-Immunofluorescence

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Description

This Cell Signaling Technology^® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as MAG (D4G3) Rabbit Monoclonal Antibody #9043.

Product Usage Information

Application	Dilution
Immunofluorescence (Frozen)	1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

MAG (D4G3) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total MAG protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

This recombinant chimeric antibody is engineered from MAG (D4G3) Rabbit Monoclonal Antibody #9043 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

The parent antibody, MAG (D4G3) Rabbit Monoclonal Antibody #9043, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg605 of human MAG protein.

Background

Myelin-associated glycoprotein (MAG), which contains five immunoglobulin-like domains, is a highly glycosylated protein (1). MAG is a component of all myelinated internodes, whether formed by oligodendrocytes in the central nervous system (CNS) or by Schwann cells in the peripheral nervous system (PNS) (2), and has several functions. A known function of MAG is its inhibition of axonal regeneration after injury. It inhibits axonal outgrowth from adult dorsal root ganglion and in postnatal cerebellar, retinal, spinal, hippocampal, and superior cervical ganglion neurons (3). Interaction between MAG and several other molecules on the innermost wrap of myelin and complementary receptors on the opposing axon surface are required for long-term axon stability. Without MAG, myelin is still expressed, but long-term axon degeneration and altered axon cytoskeleton structure can be seen (4).

Alternate Names

GMA; MAG; myelin associated glycoprotein; Myelin-associated glycoprotein; S-MAG; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A; Siglec-4a; SIGLEC4A; SPG75

Database Links

UniProt ID: P20916

Entrez-Gene Id: 4099

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.

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