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9329
MAPKAPK-2 Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

MAPKAPK-2 Antibody Sampler Kit #9329

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MAPKAPK-2 Antibody Sampler Kit: Image 1

Western blot analysis of extracts from HeLa cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).

MAPKAPK-2 Antibody Sampler Kit: Image 2

Immunoprecipitation of MAPKAPK-2 from HeLa cell extracts using MAPKAPK-2 (D1E11) Rabbit mAb (lane 2), MAPKAPK-2 Antibody #3042 (lane 3), or Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 4). Lane 1 is 10% input. Western blot analysis was performed using MAPKAPK-2 (D1E11) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody.

MAPKAPK-2 Antibody Sampler Kit: Image 3

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

MAPKAPK-2 Antibody Sampler Kit: Image 4

Western blot analysis of extracts from NIH/3T3 cells, untreated (-) or UV- and TPA-treated (+), using Phospho-MAPKAPK-2 (Thr222) (9A7) Rabbit mAb (upper) or MAPKAPK-2 Antibody #3042 (lower).

MAPKAPK-2 Antibody Sampler Kit: Image 5

Western blot analysis of extracts from various cell lines, untreated (-) or treated with UV (40 mJ/cm2, 30 min recovery; +), using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

MAPKAPK-2 Antibody Sampler Kit: Image 6

Western blot analysis of extracts from untreated or UV+TPA-treated HeLa and COS cells, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).

MAPKAPK-2 Antibody Sampler Kit: Image 7

Western blot analysis of cell extracts from MAPKAPK-2 wild type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using MAPKAPK-2 (D1E11) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). MAPKAPK-2 WT and KO MEFs were generously provided by Drs. Sandra Morandell and Michael Yaffe, David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA.

To Purchase # 9329T
Product # Size Price
9329T
1 Kit  (3 x 20 µl) $ 261

Kit Usage Information

Protocols

Product Description

The MAPKAPK-2 Antibody Sampler Kit provides an economical means of evaluating levels of MAPKAPK-2 protein phosphorylated at specific sites, as well as total MAPKAPK-2 protein levels. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Specificity / Sensitivity

Each antibody in the MAPKAPK-2 Antibody Sampler Kit detects endogenous levels of its target protein. Activation state antibodies detect only target proteins phosphorylated at indicated residues. MAPKAPK-2 (D1E11) Rabbit mAb is not known to cross-react with other MAPKAPK proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr222 and Thr334 of human MAPKAPK-2 and nonphospho peptides corresponding to residues surrounding Pro381 of human MAPKAPK-2 protein..

Background

In response to cytokines, stress, and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272, and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272, and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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